ABSTRACT
La Oficina de Naciones Unidas contra la Droga y el Delito (UNODC) en 2011 señala que "El delito facilitado por drogas (DFD) es una expresión general que abarca la violación y otras agresiones sexuales, el robo con violencia o intimidación, la extorsión de dinero y los malos tratos deliberados de ancianos o niños bajo la influencia de sustancias sicotrópicas". En este trabajo se validó un método cualitativo y rápido a partir de muestras de orina por LC/MS/MS para 39 compuestos comprendidos en los listados de sumisión química. El objetivo fue alcanzar un límite de detección un 50 % por debajo de la concentración propuesta como "Límites mínimos de funcionamiento exigidos (MRPL)" por la UNODC, para poder ser aplicado a muestras reales.
The United Nations Office on Drugs and Crime (UNODC) in 2011, states that "The Drug-facilitated crime (DFC) is a general term that includes rape or other sexual assault, robbery, money extortion, as well as the deliberate maltreatment of the elderly or children under the influence of psychotropic substances". In this work we validated a qualitative and fast method from urine samples by LC/MS/MS for 39 compounds included in the Drug-facilitated crime lists. The aim was to reach a detection limit of 50% below the proposed concentration as "minimum required performance limits (MRPL)" by UNODC in order to be applied in real samples.
Subject(s)
Humans , Chromatography, Gas/methods , Chromatography, Liquid/methods , Substance Abuse Detection/methods , Urine Specimen Collection/methods , Urine/chemistry , Sex Offenses , Substance-Related Disorders/urine , Urine Specimen Collection/statistics & numerical dataABSTRACT
The glycoconjugate profiles of epimastigotes of the Y strain and of two stocks of the tulahuen strain which differ in their infectivity have been compared. The surface location of the glycoconjugates was evidenced by labelling with glactose oxidase/NaB3H4. Fluorography of SDS-polyacrylamide gel electrophoresis showed a different pattern in the glycoprotein range for both strains and also for two lines (T0 and T2) of the Tulahuen strain. While T0 showed only one glycoprotein with Mr 45 kDa, T2 revealed three glycoproteins in the range of 25-57 kDa. Two fast components, corresponding to glycolipids were also shown. The glycoproteins were isolated with 44 percent phenol and they were purified from the aqueous phase. Alkaline borohydride treatment of the labelled glycoproteins under the conditions of beta-elimination released strongly labelled O-glycosidically linked oligosaccharides. These O-linked glycans are not of the usual type found in glycoproteins. N-acetylgalactosamine which links O-glycans to the protein in the known mucin type glycoproteins has not been detected in the T. cruzi glycoproteins (1).